Cloning and functional expression of a human lysozyme gene (hly) from human leukocytes in Pichia pastoris
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چکیده
منابع مشابه
Cloning and functional expression of a human lysozyme gene (hly) from human leukocytes in Pichia pastoris.
hly is a cDNA gene derived from human leukocytes that encodes a mature human lysozyme (abbreviated to hLY). The aim of the present study was to determine the effect of cloned hly on recombinant hLY (r-hLY) activity under optimized conditions. hly was amplified by RT-PCR and ligated into the pPIC9K plasmid. The cloned cDNA (hly) was 393 bp in length, encoding a 130 amino acid hLY with a calculat...
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ژورنال
عنوان ژورنال: Molecular Medicine Reports
سال: 2012
ISSN: 1791-2997,1791-3004
DOI: 10.3892/mmr.2012.878